《植物生理学报》 2009, 45(3): 263-266

太子参试管微块根形成及其应用初探

叶祖云¹, 王雅英², 田惠桥^3,*

1 宁德师范高等专科学校生物系, 福建宁德 352100; 2 厦门医学高等专科学校, 厦门 361008; 3 厦门大学生命科学学院, 厦门361005

通信作者：田惠桥；E-mail: hqtian@xmu.edu.cn；Tel: 0592-2186486

摘　要：

太子参组培苗单茎节段插入含45 g•L-1蔗糖的MS培养基中, 于60 µmol•m-2 •s-1光照强度下培养120 d后, 其节部位长出 不定根膨大形成与太子参体内块根相似、重量约为块根 1/20 的微块根, 平均每株可形成 1.54 个微块根。微块根移植入大田后, 其生长特性和物候期与大田块根相似, 都有低温萌芽、高温休眠的特性; 开花结果、不定根膨大、块根成熟等也相似。同时, 组培中的微块根还具有易培养、耐贮存、体积小、易移栽和繁殖系数高等优点, 在生产中具有很大的应用潜力。

关键词：太子参; 微块根; 组织培养

收稿：2008-11-03   修定：2008-11-30

资助：厦门市科技计划(3502Z20084037)。

In vitro Micro-root Tuber Formation of Pseadostellaria heterophylla Miq. and Preliminary Study of Its Application

YE Zu-Yun¹, WANG Ya-Ying², TIAN Hui-Qiao^3,*

¹Department of Biology, Ningde Teachers College, Ningde, Fujian 352100, China; ²Xiamen Medical College, Xiamen 361008,China; ³School of Life Sciences, Xiamen University, Xiamen 361005, China

Corresponding author: TIAN Hui-Qiao; E-mail: hqtian@xmu.edu.cn; Tel: 0592-2186486

Abstract:

When sterile stems of ‘Taizishen’ (Pseadostellaria heterophylla) were put in MS medium with 45 g·L^-1sucrose in 60 µmol·m^-2·s^-1 illumination for 120 days, adventitious roots from nodes would enlarge and form micro-root tubers which were same with root tubers in vivo, but had a 1/20 weight of the latter. Each plantlet averagely produced 1.54 micro-root tubers. When the micro-root tubers plateded in field, they displayed same growth features and phenophase with root tubers in vivo, such as germination in low temperature and dormancy in higher temperature. The plants from micro-root tubers also could flower and set up seeds, and its adventitia roots could enlarge and form mature root tubers. The micro-root tubers were small in size, easy of culture and transplant, convenience of storage and higher ability of propagation, which had great potential application in production of P. heterophylla.

Key words: ‘Taizishen’ (Pseadostellaria heterophylla); micro-root tuber; tissue culture